recombinant human podocin  (Novus Biologicals)

Journal: Scientific Reports

Article Title: Autoantibodies against nephrin and podocin are associated with disease severity and steroid dependence in adult-onset nephrotic syndrome

doi: 10.1038/s41598-026-43612-7

Figure Lengend Snippet: Serum anti-nephrin and anti-podocin antibody levels among glomerular disease groups. Scatter plots show the distribution of anti-nephrin (left) and anti-podocin (right) antibody levels in patients with MCNS, FSGS, PLA2R-MN, NELL1-MN, and controls. Horizontal dashed red lines indicate the ELISA cutoff values (0.2 AU/mL for anti-nephrin and 90 AU/mL for anti-podocin), determined as the upper limit of the control group. Patients double-positive for both antibodies are indicated by open circles of the same color. Anti-nephrin antibodies were predominantly detected in MCNS and FSGS, whereas anti-podocin antibodies were more frequently observed in NELL1-MN.

Article Snippet: Recombinant human Nephrin (Sino Biological, #17,757-H08H) and Podocin (R&D Systems, #9287-PO) were coated onto ELISA plates at 100 ng/well in coating buffer (BioLegend, #421,701) and incubated overnight at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay, Control